FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698160
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCC672423.9596975550007065No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTT440152.5919230225656005TruSeq Adapter, Index 4 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTG136690.8049300419277335TruSeq Adapter, Index 4 (95% over 24bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTT87580.5157346775333302TruSeq Adapter, Index 4 (96% over 27bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCG59150.3483181796768267No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGT48290.28436660856456397No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTG44350.26116502567484806TruSeq Adapter, Index 4 (95% over 24bp)
GATGGAGACTACCCCATCGACACCTACAGCGACGGCCTCAGGCCCCCGTACCCCACTGCAGACCACATGCTGGCC28880.1700664248362934No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTG28610.16847646864841948TruSeq Adapter, Index 4 (96% over 28bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG24160.14227163518160832No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAA24060.1416827625194328TruSeq Adapter, Index 4 (96% over 28bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAA22470.13231968719084186TruSeq Adapter, Index 4 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)