FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004048329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92070
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTT16101.7486694906049747RNA PCR Primer, Index 47 (96% over 29bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCC13031.4152275442598024No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTT3060.33235581622678395RNA PCR Primer, Index 47 (96% over 29bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTG2090.22700119474313024RNA PCR Primer, Index 47 (96% over 26bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG2050.22265667426957747No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGT1470.15966112740306287No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCG1320.14336917562724014No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT1280.1390246551536874No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTG1200.13033561420658196RNA PCR Primer, Index 47 (96% over 26bp)
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC930.10101010101010101No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)