FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764438
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTT88341.1556202072633752Illumina PCR Primer Index 7 (96% over 26bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCC81991.072552646519404No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTG31410.4108900918060065Illumina PCR Primer Index 7 (95% over 23bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTT15690.20524882331856867Illumina PCR Primer Index 7 (96% over 26bp)
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTG9380.12270452279975616Illumina PCR Primer Index 7 (95% over 23bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCG8420.11014627739594315No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)