FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114833
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTT999164.72453380479688Illumina PCR Primer Index 10 (96% over 26bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCC873554.130586197586287No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTG297151.4050754835015342Illumina PCR Primer Index 10 (95% over 23bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTT145110.686153469328311Illumina PCR Primer Index 10 (96% over 26bp)
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTG96170.4547403979415869Illumina PCR Primer Index 10 (95% over 23bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGT76350.36102141398398835No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCG75960.35917729674163396No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA71130.33633861397093767Illumina PCR Primer Index 10 (96% over 27bp)
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTG56880.2689574070387591Illumina PCR Primer Index 10 (96% over 27bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA43830.2072504070061324Illumina PCR Primer Index 10 (96% over 27bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG41980.19850267136932326No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA32600.15414928743782605Illumina PCR Primer Index 10 (96% over 27bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT24980.11811807362567162No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGC21790.10303414028436289Illumina PCR Primer Index 10 (95% over 24bp)
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC21200.1002443218920832No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)