FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322969
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCC971477.343104789303453No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTT647984.897922778235922Illumina PCR Primer Index 6 (96% over 26bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTG247701.8723038861832741Illumina PCR Primer Index 6 (95% over 23bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTT111580.8434060057340724Illumina PCR Primer Index 6 (96% over 26bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG82450.6232194405159909No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTG80340.6072704651431742Illumina PCR Primer Index 6 (95% over 23bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGT76250.5763551526906526No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTG46840.3540521357643301Illumina PCR Primer Index 6 (96% over 27bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAA33970.2567709447462488Illumina PCR Primer Index 6 (96% over 27bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAA28810.21776776326580594Illumina PCR Primer Index 6 (96% over 27bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG25580.19335298106002485No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAA23400.1768748927601478Illumina PCR Primer Index 6 (96% over 27bp)
AATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTC16750.12660916468942204No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT16040.12124244785781071No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG15630.1181433578564577No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGC15090.11406162956199276Illumina PCR Primer Index 6 (95% over 24bp)
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG15090.11406162956199276No Hit
ACCAGAAGGACCCTGGTGTCCTTGACCGCATGATGAAGAAACTGGACACCAACAGTGATGGTCAGCTAGATTTCT14500.10960196346248477No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAA13570.10257232028868402Illumina PCR Primer Index 6 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)