FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798440
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC132341.6574820900756473No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTT92431.157632383147137TruSeq Adapter, Index 7 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTG31860.39902810480436857TruSeq Adapter, Index 7 (95% over 24bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTT12770.15993687690997446TruSeq Adapter, Index 7 (96% over 27bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG10010.12536947046741145No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG9020.11297029206953559No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTG8210.10282550974400079TruSeq Adapter, Index 7 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)