FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1798278
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCC763484.2456171960064015RNA PCR Primer, Index 32 (95% over 21bp)
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTT577993.2141304069782315RNA PCR Primer, Index 32 (96% over 32bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTG197811.0999967746922334RNA PCR Primer, Index 32 (96% over 29bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTT75440.4195124446831913RNA PCR Primer, Index 32 (96% over 32bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG55990.31135341699114377RNA PCR Primer, Index 32 (95% over 22bp)
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTG52920.2942815293297254RNA PCR Primer, Index 32 (96% over 29bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT50970.28343782218322194RNA PCR Primer, Index 32 (95% over 23bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA39500.21965458065994248RNA PCR Primer, Index 32 (96% over 33bp)
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTG37260.2071982196301128RNA PCR Primer, Index 32 (96% over 33bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG35440.1970774262933762No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA31230.17366614060784819RNA PCR Primer, Index 32 (96% over 33bp)
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTCTTCTGCTT21930.12194999883221615TruSeq Adapter, Index 4 (96% over 25bp)
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA21570.11994808366670782RNA PCR Primer, Index 32 (96% over 33bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT20130.11194042300467447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)