FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816624
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCC504006.171751013930524No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTT469215.745728756441153Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTG112051.372112502204197Illumina Paired End PCR Primer 2 (95% over 24bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTT58690.7186906091420286Illumina Paired End PCR Primer 2 (96% over 27bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCG44400.5437018750367366No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTG41520.508434726385705Illumina Paired End PCR Primer 2 (95% over 24bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGT36850.4512480652050393No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAA27960.34238523482043143Illumina Paired End PCR Primer 2 (96% over 28bp)
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTG21780.26670781167342617Illumina Paired End PCR Primer 2 (96% over 28bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG20950.2565440153608025No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAA17660.2162561962420894Illumina Paired End PCR Primer 2 (96% over 28bp)
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAA13320.163110562511021Illumina Paired End PCR Primer 2 (96% over 28bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATGTCGTATGCCGTCTTCTG11630.14241560375399204TruSeq Adapter, Index 1 (95% over 23bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT11480.14057877309508415No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC11040.135190736495621No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCG9050.11082211642077627No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGC8520.10433198142596838Illumina Paired End PCR Primer 2 (96% over 25bp)
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATGTCGTATGCCGTCTTCTGCTT8410.1029849722761026TruSeq Adapter, Index 1 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)