FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004048343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767105
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCC26166034.11006315954139No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTT480316.2613331942823995Illumina PCR Primer Index 6 (96% over 26bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTG325284.2403582299685185Illumina PCR Primer Index 6 (95% over 23bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG165242.1540727801278834No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGT127301.659485989532072No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTG46390.6047412023125909Illumina PCR Primer Index 6 (96% over 27bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAA38020.4956296726002308Illumina PCR Primer Index 6 (96% over 27bp)
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG32310.4211939695348095No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAA27030.35236375724314145Illumina PCR Primer Index 6 (96% over 27bp)
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG24300.3167754088423358No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACG22750.2965695700067136No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTG19020.24794519655066777Illumina PCR Primer Index 6 (95% over 23bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATATCGTATGCC18310.23868961876144726No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA15370.2003637050990412No Hit
ATGCACTGCAGGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTA14130.1841990340305434No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCAC13580.17702922025016132No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCT13220.17233625123027485No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAA12680.16529679770044517Illumina PCR Primer Index 6 (96% over 27bp)
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAA12460.16242887218829236Illumina PCR Primer Index 6 (96% over 27bp)
ACACCACCCGCCGGCCCTGCGCGCCCAGCCTGGCCGCGAAGCGAGCCATAACCTCAGCCCAGCGCGCAGCACCGG11950.15578049941011987No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCA11570.1508268098891286No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTA11240.14652492162089936No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTG11080.14443915761206094Illumina PCR Primer Index 6 (96% over 27bp)
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCC11040.1439177166098513No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTA10950.14274447435487972No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTT10810.1409194308471461Illumina PCR Primer Index 6 (96% over 26bp)
TGAGAGAGACAGAAGGAGGACGATGACCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCT9870.12866556729522033No Hit
GTCCTGCACTCTGAAGGGCAGAGATCTTCTCTTTAGCTCCACCTTTTCCTCCAGCAATAATTGCCCCGGCATGAC8840.11523846148832298No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACG8570.11171873472340813No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA7700.1003773929253492Illumina PCR Primer Index 6 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)