FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083236
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCC181341.6740580999892913RNA PCR Primer, Index 32 (95% over 21bp)
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTT125021.1541344637733606RNA PCR Primer, Index 32 (96% over 32bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTG43890.40517486494171173RNA PCR Primer, Index 32 (96% over 29bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG35280.3256908005273089No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC18140.16746119959085554No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT18050.16663035571195936No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTT14920.13773545192368053RNA PCR Primer, Index 32 (96% over 32bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13060.12056467842649247No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG12100.11170234371826637RNA PCR Primer, Index 32 (95% over 22bp)
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG11270.10404011683511256No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)