FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173501
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCC159771.3614815837395962No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTT104260.888452587598988Illumina PCR Primer Index 10 (96% over 26bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTG44840.38210448904602556Illumina PCR Primer Index 10 (95% over 23bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTT14880.12680006237745003Illumina PCR Primer Index 10 (96% over 26bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCG13820.11776726223497039No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG13510.11512559426877353No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)