FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004048350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553793
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCC15186527.422701262023896No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTT423927.65484576366982Illumina PCR Primer Index 6 (96% over 26bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTG262884.74690001498755Illumina PCR Primer Index 6 (95% over 23bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG112082.0238609010948134No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGT93871.695037676532567No Hit
GGCTGGTGGATCACTTGAGGTCAGGAGTTCAAAAACAGCCTGACCCACATGGTGAAACCCCGTATCTACCTGTCT39150.7069428468759988No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTG38180.6894272769789435Illumina PCR Primer Index 6 (96% over 27bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAA31280.5648319859586524Illumina PCR Primer Index 6 (96% over 27bp)
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTG24150.43608351857101835Illumina PCR Primer Index 6 (95% over 23bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAA23770.42922174892062553Illumina PCR Primer Index 6 (96% over 27bp)
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG19940.3600623337600872No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG18200.32864265167670953No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTT17780.32105859048417007Illumina PCR Primer Index 6 (96% over 26bp)
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAA13880.2506351651248752Illumina PCR Primer Index 6 (96% over 27bp)
ATCAGTACCAGAGCTTGAAGAACAGGATTCCACCCAGGCAACCACACAACAAGCCCAGCTGGCGGCAGCAGCTGA13860.2502740193538019No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAA12270.22156293055347395Illumina PCR Primer Index 6 (96% over 27bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATATCGTATGCC11500.2076588183671516No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTG11260.20332506911427192Illumina PCR Primer Index 6 (96% over 27bp)
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCT10570.19086554001224285No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACG10080.1820174686209468No Hit
GAAGAAGGTAAGGACAAAGAGGGAGCCTGTGATAACAGGAAAGGCCCACTCCCCGTTCTGAGCCAGCCACCTGCA8130.14680575594129935No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCC7660.13831883032107664No Hit
CCTTCAGACCTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTG7460.13470737261034357Illumina PCR Primer Index 6 (95% over 23bp)
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA7340.13254049798390372Illumina PCR Primer Index 6 (96% over 27bp)
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT6670.12044211465294795No Hit
TGCTACCACTGTACTGTCGCGTGCCATTGCTGAGCTGGGCATCTATCCAGCTGTGGATCCTCTAGACTCCACCTC6310.11394149077362842No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA6290.1135803450025551No Hit
TGAGAGAGACAGAAGGAGGACGATGACCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCT6220.11231633480379853No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGT6220.11231633480379853No Hit
ATGCACTGCAGGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTA6170.11141347037611525No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)