FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127446
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCC1000488.873861808015638No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTT473544.200112466583765TruSeq Adapter, Index 4 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTG185571.6459324881191648TruSeq Adapter, Index 4 (95% over 24bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCG70290.6234444931287174No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTT51740.4589133315475863TruSeq Adapter, Index 4 (96% over 27bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGT51630.45793767506381683No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTG39020.34609196360623923TruSeq Adapter, Index 4 (95% over 24bp)
GTCCTCCATAAATAAATCTGTACAGAGTTGGAAAGTAAGACCATGGCATATACAGAAGGAAAGAGCACAGTGTTA34800.3086622330470816No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTG33600.2980187077695961TruSeq Adapter, Index 4 (96% over 28bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAA27870.24719587456960243TruSeq Adapter, Index 4 (96% over 28bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAA27690.2455993457779796TruSeq Adapter, Index 4 (96% over 28bp)
CTCCTCCATAAATAAATCTGTACAGAGTTGGAAAGTAAGACCATGGCATATACAGAAGGAAAGAGCACAGTGTTA26800.23770539786384445No Hit
GACGTCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCG24070.2134913778575648No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG22720.20151741192039352No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAA18930.16790161125233494TruSeq Adapter, Index 4 (96% over 28bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATATCGTATGCC16500.14634847256542663No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT13290.11787704244815272No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCG13030.11557094530469751No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATATCGTATGCCGTCTTCTGCTT12660.1122891916774728RNA PCR Primer, Index 36 (96% over 27bp)
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAA12160.10785438947852048TruSeq Adapter, Index 4 (96% over 28bp)
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC11630.10315349914763101No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)