FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004048355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581789
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCC31510.5416052898903211No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTT18190.3126563066678813Illumina PCR Primer Index 7 (96% over 26bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTG8670.14902309943983127Illumina PCR Primer Index 7 (95% over 23bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG8460.14541354339803605No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)