FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001052
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCC133961.3381922217826847No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTT55060.5500213775108587Illumina PCR Primer Index 10 (96% over 26bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTG25120.2509360153118919Illumina PCR Primer Index 10 (95% over 23bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG15930.15913259251267667No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCG10170.10159312403351674No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)