FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004048362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655638
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC145062.212501410839518No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTT71421.0893206312019743TruSeq Adapter, Index 1 (96% over 28bp)
GAGGAAGCCCTCTGTACGAAAAGACCACAGGGCCCATGCCGCCCAGTTTGCTCTGGAGAATCTCACGCAGAAGGC17530.2673731540880791No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTG10680.16289476814949713TruSeq Adapter, Index 1 (96% over 25bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG9850.1502353432839463No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)