FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797636
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC38600.4839300131889734No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTT26750.33536600654935333RNA PCR Primer, Index 33 (96% over 28bp)
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAACCAAAGCTAAGACCCCCGAAACC15400.19307052339663705No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG8700.10907230867212613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)