FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707169
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTAATTCCGACGGGTTCTTGGTCTCAAAGGTTAAAATCTCCAGGTCGGTGTCCGCGGCCTCTGGGGCCCCGA38230.5406062765760377No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT28280.399904407574427TruSeq Adapter, Index 11 (96% over 27bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC28190.3986317273523019No Hit
ATGCTAATTCCGACGGGTTCTTGGTCTCAAAGGTTAAAATCTCCAGGTCGGTGTCCGCGGCCTCTGGGGCCCCGA8380.11850066957120575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)