FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004048403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593178
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC35340.5957739498093321No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTT16480.2778255430916184RNA PCR Primer, Index 39 (96% over 29bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG6750.11379383591434611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)