FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959956
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC95640.9962956635512461No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT57080.5946105863185396No Hit
GTACATGGGACCGTCGATTACGGTCCGATCTCGGCGAAGAAGCTCGACCC37520.39085124734883686No Hit
GATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTC26500.2760543191562947No Hit
CTCATCGTTCGGGGGAGACTGAAGATACCTTCATCGCTGACCTGGTTGTG24880.25917854568334386No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC23480.24459454391659616No Hit
ACGCAGAGTACATGGGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAA22310.23240648529724278No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG21190.2207392838838447No Hit
GATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTA20960.21834334073645043No Hit
CTTTCTCCTCTTCAGATTCAGATTTAAAATGATGGTTGTTTTTCCGAGCT20170.21011379688235712No Hit
TGATCAGCGTAAGCGCCATGGCCATAGCTTTCCTGACCCTGGGCTACTTC19630.20448853905804015No Hit
GGCATAAGCGTGCTTGACTGCGAGACTGACAAGTCGAGCAGGTACGAAAG19150.19948830988086955No Hit
GAGTTCCCCAAACAGGTAGTGTCATTTCACACCTCCGTGCCTTCGTGCAC19040.19834242402776794No Hit
TCTGCACCTCCAGCGCCGACAGGTCGACGACCGACAGCAGCTTGGCGTCG17700.18438345090816663No Hit
CCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTT17500.18230002208434554No Hit
TCATCGTCCAGCCCGGCGGCAAGGTGAGCTGGGACTTCACCGCCGACGCG16820.17521636408335384No Hit
GCTGGTGGGCTGACTGGCTGTGATTTCTACTTTCGTGGTTCCCCAGAAGG15860.16521590572901257No Hit
ACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTG15480.1612573909637525No Hit
GAGTACATGGGTAGCCCGGGGTTTGTTGTGGTCCTTTGGAGATTTCTTTG15280.15917396213993143No Hit
GATCGGAGGGGTCAACAGACAAAGAGGAGTCAGCATCGCCAGTGGAAGAA15120.15750721908087453No Hit
TCACAGCACAGTAACAAAGTTATTAGGAAAACAGGACTACCACAACCAAA14840.15459041872752502No Hit
CTGTGGGATCTTCCTTCATGGCTGCCATCCACAGTTTCAGTTTTGGAGTG14600.1520903041389397No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCTCAC13850.1442774460496106No Hit
CGCAGGTGTTCTTCAACGGTGCCAACGTCCGGCAGGTGGACGTGCCCACG13490.14052727416673264No Hit
GTGCTTAGGAAGGTTATATAATCTGCAGAGTCCACATATCAGCCACTTCC13460.14021475984315948No Hit
AGATTAGCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAG13140.13688127372504574No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG12960.13500618778360676No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12690.13219355887144826No Hit
GGCCACCACCCCGGAGGCGGCGAGCAGCACCATGCCGACGGCGGTCACGC12540.13063098725358244No Hit
ATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCTTGT12150.12656830104713135No Hit
CATCTGGTCCACACAGATCCTTAAAGTAATCTTCATTCTTGAAGACAAAC12150.12656830104713135No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11940.12438070078211919No Hit
GCTCCTGCAGGATTTCAGCCGCAATCTCTCGGCCAAGTCCTCCGCGCTCT11570.12052635745805017No Hit
CAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCTCACCC11470.11948464304613962No Hit
ATGGAATGGAACGGAATGGAATTGAATGGTATGGAATGGAATGCAATGGA11380.11854710007542012No Hit
GGAGGGAGTGGCTGCAAATTGCTGCTTCCACCTGCTCCACCACCCGTCGT10730.11177595639800157No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGC10660.1110467563096642No Hit
CTCTCCTTCCCCACCCCAGTATCTCCTTGCCTCAAGGGTTTCCTGTTACT10620.11063007054489997No Hit
GACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTG10510.10948418469179837No Hit
CTGTTTTATAGCCGTACTACCATGGAAGTCTTTCGCAGAGAGATATGGTA10510.10948418469179837No Hit
GTCACATGGCAGGCCGTTGCCGTGCGCTCTTATCCTGTAAACAGAAAACC10340.10771327019155044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.10729658442678623No Hit
CACTCGGCCCATCTTGGTGCGGATGTTTCGTAGGAGGAAGAAGGCAGCTG10170.10594235569130252No Hit
CACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAGA10110.10531732704415618No Hit
GTACATGGGTGTGTGTCAGTTTGCAAACAAGAACTGCCCTTTTCTCTTTG10020.1043797840734367No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)