FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004048455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482295
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC184240.7422163763775055No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC96690.38951857051639716No Hit
AATCGCTTCTTTCCTCATTTGTCTCACCTTATTCAGAACTTCAGATAATG95270.38379805784566295No Hit
GCTTGCACCCCATCATTTAATCTTCCAGCACCGGGCAGGAGTCACACCCT93080.374975577036573No Hit
GCGTCGAGACGGTTCCGCGCCTTCCTGCGGGAGCGGCCAAGCCAGAAGAG83080.33469027653844524No Hit
TCACTACACATTCCTATTTTGCCCGCTCTGTTAACTCTGGCGCTGTTACT71250.28703276604916017No Hit
TCCTTATTGACGACAGTTTTGTCCAAGGGGATATCCACAGAGTACCTTGT68840.2773240086291114No Hit
CACTTCACGCGCGGCTACGGGCTGGTGTTCGGCATGAGCGAGCGCAAAGC66310.267131827603085No Hit
ATACAAGTACATCCAGGAGCTATGGAGAAAGAAGCAGTCTGATGTCATGC57520.23172104846523076No Hit
GCGATATGACAAGTTAACAAGACGGCGCAGGTTGATGTTTCCAATAAACA51080.2057773149444365No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG44020.17733589279275833No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA43410.1748784894623725No Hit
CCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCT41750.16819112957968332No Hit
GTACTGGTTTGCCACTGAACAAGGCCAGGCGGTGGATCTGAACAGCCTGA34860.1404345575364733No Hit
GCGATATGACAAGTTAACAAGACGGCGTAGGTTGATGTTTCCAATAAACA32590.1312897943233983No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG30490.12282988121879149No Hit
GTCATGACCATTCCGTACCAGCCCATGCCGGCCAGCTCCCCAGTCATCTG29780.11996962488342441No Hit
CGCCCGGCCGGCGACCTCACCAAACCGCGACGGAAGTGCTGACATAGTTC27700.11159028237981385No Hit
GGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATC27400.11038172336487No Hit
GTACAACACAGCAATGTGGTCATCAATCTTATTGGACGAGACTGGGAAAC25550.10292894277271639No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)