FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences676037
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAATAGTAGTGTTTCTTGGTTTCTGATTCAACCATAGATGAAAGAACTC90491.338536204379346No Hit
GAGTTGACCTTGCGTTTCAAAATGGACGGCACCATGATTTATCGTTTGGC57390.8489180325928906No Hit
GCAATGATCTTGATCTTCATTGTGCTGGGTGCCAGGGCAGTGATCTCCTT53490.791228882442825No Hit
CGGCGCTGGTAGACGCGCGCCTGGCGGCGCGCGCGCCGCAACTCCGCGGC47130.6971511914288715No Hit
TATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTA38410.5681641685292372No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC28090.4155098019782941No Hit
TGGCGCTGGTAGACGCGCGCCTGGCGGCGCGCGCGCCGCAACTCCGCGGC24600.3638854086388763No Hit
ACGCAGAGTACATGGGGAAGCTGGGGAGATTTTTAAGGTCGAGCCAACAG22310.33001152303794024No Hit
CTAAAACTCTATTCTTCTCCCTGGAGTCCAGATCCCCATTCCTTGCAACA14900.22040213775281528No Hit
GCCTTCTTGAAGTAGCAGAGCCGACACACGGAGTGGTACTTGTCTGCTCC14290.21137896298575373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13690.20250370911651283No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCT13370.19777024038625102No Hit
ATGCAGAGTACATGGGGAAGCTGGGGAGATTTTTAAGGTCGAGCCAACAG13190.1951076642254788No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12140.17957596995430725No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC11920.17632171020225224No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11100.16419219658095635Clontech Universal Primer Mix Long (96% over 26bp)
GTCGTAGAGATCACAACGGACGAGGGCCACATCGGCATCGGCAACGCCGG10720.1585712024637705No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10470.15487318001825345Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9640.1425957454991369Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATAGTTGCTTATTGTTCATTAAGCATAAGTACACAATGCCGCAGTTAAT9430.13948940664490259No Hit
AATCCTTCCACCTCAGCCTCCCAAAGTGCTGGGCTTACAGGTGTGAGCCA7380.10916562259166288No Hit
CTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT6860.10147373590498744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)