FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004048481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690145
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC7662311.1024494852531No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT101611.4722992994225852No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA74571.0804975765962226No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA74171.0747016931224598No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA52770.7646219272761521No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT52630.7625933680603351No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG49980.7241956400466568No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG42950.6223329879952764No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA35270.5110520252990314No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA33210.48120322540915317No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT32640.47294409145904115No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA32060.46454006042208523No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT29320.4248382586268103No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT26830.38875888400263714No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG25550.3702120568865963No Hit
GCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTC24730.3583304957653826No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCTGTC21520.3118185308884365No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCCTGTCTCTTAT19770.2864615406907244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17630.25545356410609366No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCCT17460.25299031362974445No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC15400.2231415137398663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14090.2041599953632932No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGCTGTCTC11370.16474798774170643No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC10960.1588072071810996No Hit
GTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCA10400.15069297031783177No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG10380.1504031761441436No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA10380.1504031761441436No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCT9530.13808692376239776RNA PCR Primer, Index 1 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT9000.1304073781596621TruSeq Adapter, Index 1 (95% over 22bp)
GTATCAACGCAGAGTACATGGGGCAGGCCTCTGCAGTCGACGGGCCCGGT8380.12142375877532982No Hit
ATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATC8270.11982989082004505No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA7010.10157285787769238No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)