FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695916
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC22780213.432386981430684No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG193291.139738053063949No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG140360.8276353309951672No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT135060.7963837831590717No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA128590.7582333087251962No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA117990.695730213053005No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA81590.48109694112208384No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA80500.4746697360010755No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT78920.46535323683484325No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG66070.38958297462846037No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA63560.37478271329476226No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT62690.3696527422348748No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG59140.3487201017031504No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG49460.2916418030138285No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA47090.2776670542644801No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT37610.22176805926708634No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG32750.1931109795532326No Hit
GCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTC30480.17972588264984823No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT30160.17783899674276318No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC29420.1734755730826291No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC29340.17300385160585785No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG24840.14646951853747472No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCTGTC23730.13992438304727356No Hit
AGGGCTTCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC23030.1357968201255251No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCCTGTCTCTTAT22540.13290752608030115No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCCT22000.12972340611209518No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCAC21890.1290747890815347No Hit
ATGCACTGCAGGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGC21390.12612652985171435No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA21370.12600859948252155No Hit
GTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCA20930.12341413136027964No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG20720.12217586248375509No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT20210.11916863806933835RNA PCR Primer, Index 17 (95% over 23bp)
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC18790.11079558185664855No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)