FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004048494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531627
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC158922.9893139362748693No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT21610.40648800756921677No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA19330.36360079529444517No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA19020.35776963923954197No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA18240.34309769819817276No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA13120.24678957238815938No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT9980.18772560460623708No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT9030.16985593282508224No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA8140.1531148718932635No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG7060.1327998766052138No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA6320.11888034279673529No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT6230.11718742652273118No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT5370.10101067101558048No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)