FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004048496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5329
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT81615.312441358603865No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT3276.136235691499343TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT1913.584162131732032RNA PCR Primer, Index 1 (95% over 23bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC901.688872208669544No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC811.5199849878025897No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT571.0696190654907112No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGAGCTCGTATGCCGT400.7506098705197973No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATGTCGTATGCCGT350.6567836367048226No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT290.544192156126853TruSeq Adapter, Index 1 (96% over 26bp)
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG240.4503659223118784TruSeq Adapter, Index 1 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACTAGGCATGATGTCGTATGCCGTCTT170.31900919497091385No Hit
AGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATC140.262713454681929No Hit
CATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATC130.24394820791893415No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC130.24394820791893415No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA130.24394820791893415No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC120.2251829611559392Illumina PCR Primer Index 5 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGAGCTCGTATGCCGTCTTCT110.20641771439294426RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATATCGTATGCCGT110.20641771439294426No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATGTCGTATGCCGTCTTCT90.1688872208669544RNA PCR Primer, Index 5 (95% over 21bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA70.1313567273409645No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGAGGTCGTATGCCGT70.1313567273409645No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA60.1125914805779696No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT60.1125914805779696No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGCTCTCGTATGCCGT60.1125914805779696No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGCTCTCGTATGCCGTCTT60.1125914805779696No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGAGCTCGTATGCCGTCTT60.1125914805779696No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)