FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832240
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC34655718.914388944679736No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA404502.2076802165655156No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG380002.073964109505305No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA170080.9282626730122692No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC89660.4893463738374885No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA71690.3912697026590403No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG66170.36114264506833166No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC59300.323647557088591No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA52080.2842422390079902No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA49510.27021569226738856No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATC46010.25111339125878707No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG44350.24205344278042176No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT41710.2276448500196481No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG41460.22628039994760513No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA40230.2195673055931537No Hit
AGGGCTTCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC39220.21405492730210016No Hit
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC34460.18807579793040213No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCAC32240.1759594812906606No Hit
TGTCCATGGCATCCATAGCGCCTGTCTCTTATACACATCTCCGAGCCCAC29900.16318822861633847No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC28360.1547832161725538No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA28250.15418285814085492No Hit
ATGCACTGCAGGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGC25810.14086582543771559No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT24580.1341527310832642No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA22780.12432869056455487No Hit
TGTCCATGCATCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA22080.12050823036283456No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA21740.11865257826485613No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC21520.11745186220145834No Hit
TGGTGTCTGTGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA20790.11346766799109285No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA19030.10386193948391041No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)