FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899837
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC1865169.817473814858854No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG194221.0222982287427815No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG134340.7071132944563139No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA82800.43582686304140833No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA48000.2526532539370483No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT47730.2512320793836524No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA39340.20707039603923916No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA38480.2025436919062004No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG35780.1883319463722414No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA27300.14369653817669623No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG27010.1421700914341599No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC25630.13490631038346973No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG22010.11585204414905068No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA21810.1147993222576463No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT21220.11169379267800343No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG20570.10827244653093923No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA20290.10679863588297313No Hit
AGGGCTTCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC19830.10437737553274308No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT19380.10200875127708324No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA19340.10179820689880238No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)