FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737965
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC1597869.193856032773963No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT165620.9529535980298798No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG123440.7102559602753795No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT68290.3929308127609014No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA67120.38619880147183633No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA61400.35328674628085144No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA59560.34269965160403115No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA41620.23947547850503317No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT35890.20650588475602216No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCT34570.1989107950965641No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA32370.1862523123308007No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG30670.17647075746634713No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT29910.17209782705635612No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCA27360.15742549475967582No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG25440.14637809161864593No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT24710.14217777688273356No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT21840.1256642107292149No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC21520.1238229768723766No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG20280.11668819567712814No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA19750.11363865210173968No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT18120.10425986714346952No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)