FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1660364
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC25326715.253703404795575No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA269551.6234391976699087No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG157920.9511167430756147No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA89380.5383156946308159No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT80360.4839902575579812No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA70240.4230397671835815No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA66390.39985208062810323No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC55170.3322765369521382No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA43730.26337598261586015No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG40920.24645198281822542No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT36050.21712106501947767No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG36030.21700060950490377No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC35660.21477218248528634No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT34730.20917100105759942No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA33700.2029675420570429No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA29710.17893666689954732No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG29490.17761165623923428No Hit
TGTCCATGGCATCCATAGCGCCTGTCTCTTATACACATCTCCGAGCCCAC28470.17146842499596474No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28450.1713479694813908No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA28150.16954113676278215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23240.13996930793488657No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT22490.13545222613836483No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG22490.13545222613836483No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATC22480.13539199838107788No Hit
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC22430.1350908595946431No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCAC19670.11846799858344316No Hit
GCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTC19360.1166009381075475No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT17990.10834973535923448No Hit
ATGCACTGCAGGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGC17300.1041940201064345No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC17090.10292923720340841No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)