FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633561
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC32165719.690541093965884No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA192271.176999206029037No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG187691.148962297704218No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA113750.6963315113423986No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG69740.42692008440456153No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC56790.34764542003635No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT51570.3156906904609011No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA51560.31562947450386No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC44410.27186006521948064No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA43300.2650650939879196No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG41780.25576026851767397No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33940.20776695819745944No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCAC32490.19889064442650137No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA31260.1913610817104473No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA29690.18175017645499617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25600.15671285002519036No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA25280.1547539393998755No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT25100.15365205217313585No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA24720.15132584580557445No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG23040.14104156502267134No Hit
ATGCACTGCAGGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGC22990.1407354852374659No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG22080.13516483314672667No Hit
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC22040.13491996931856234No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT21970.1344914576192747No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC21020.12867594170037114No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATC17310.10596482163812677No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)