FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004048536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2721165
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC48213417.717925961858246No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG428621.57513417966202No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC231220.8497095912963749No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA187460.6888961161855308No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA138960.510663631202077No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG115190.4233113390771967No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC71860.26407806950331936No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT70290.2583084818450921No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA68730.25257564315284076No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG64220.23600185949767838No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA58100.21351149232038485No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA55320.20329527977906522No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCAC53910.19811367557645349No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC52460.19278507550993784No Hit
AGGGCTTCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC50070.18400207264168106No Hit
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC42330.15555837297628036No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA42130.15482339365676098No Hit
ATGCACTGCAGGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGC41160.1512587439570919No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT39180.14398244869384988No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA39090.14365170800006616No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT31680.11642072421187248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29810.10954866757436613No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC29510.10844619859508703No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT28980.10649850339836063No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA28260.1038525778480908No Hit
TTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCA27260.10017768125049381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)