FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470816
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC1417769.6392750690773No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG88080.5988512499184127No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG70990.48265724604573246No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT49040.3334203598546657No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA41480.2820203206927311No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT34350.23354382873180599No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA31560.2145747666601397No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG30240.2056001566477384No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG30160.20505624088941105No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACT23850.1621548854513413No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA21850.14855699149315754No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGC21270.14461360224528425No Hit
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGG21030.1429818549703022No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA20980.1426419076213476No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA20900.14209799186302025No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT20040.13625089746100125No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC19650.1335993081391554No Hit
GCACTAGCCTTTACGGGCCGGCGCCTTCCAGCACCTGGTTGTGGACGCCC19090.12979189783086395No Hit
TTCCAGCACCTGGTTGTGGACGCCCTTGGCAGGCTTCGGCCGTCCGGCGT18350.12476067706633595No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA16500.11218262515501598No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG16360.11123077257794312No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA16220.11027892000087026No Hit
GGCTAGTGCCGACCTGCTCCCGGTGCTTCCACCCAAGGCCCAACGTACCC16110.10953103583317014No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGG15750.10708341492069709No Hit
CCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAA15390.10463579400822401No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA15260.10375193090094206No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACG14870.10110034157909623No Hit
ATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTC14710.10001251006244152No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)