FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225676
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC20370216.619563408274292No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA134701.0989853762331971No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG85660.6988796386647042No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAA73080.5962424001122646No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA64200.5237925846634837No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATT56670.4623570992660377No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATG52640.4294772843720526No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAA47040.38378821156651516No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTT45290.36951037631478467No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC41470.33834390165100725No Hit
CTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACT36680.2992634268762707No Hit
TTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCC33580.2739712615732053No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG32270.2632832820419099No Hit
GAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGA31790.2593670758014353No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC27730.2262424980174206No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA27640.22550820934733162No Hit
ATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAA26040.21245418854574943No Hit
CGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT24300.19825794092402887No Hit
GTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAA23100.18846742532284225No Hit
GTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGG22820.18618297168256537No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG21870.17843214683162598No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTT20260.16529653840003394No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA19140.15615872383892643No Hit
CCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTG18920.1543637959787089No Hit
GAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAAC18740.1528952186385309No Hit
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC18410.1502028268482046No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTA17930.14628662060772996No Hit
GTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGC17670.14416534222747285No Hit
GTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACGCAAGTAGTC17440.1422888267372454No Hit
AGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTC17400.14196247621720584No Hit
GTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGA17100.1395148473169092No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCAC16850.13747515656666198No Hit
GAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTT16540.13494594003635546No Hit
GTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGC16030.13078497090585114No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGG15950.13013226986577203No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG15490.12637923888531716No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCAC15410.12572653784523805No Hit
GAACCGCACTCCTCGCCACACGGGATTCTCACCCTCTATGACGTCCTGTT15350.12523701206517873No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC14960.12205509449479308No Hit
AGGGCTTCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC14910.12164715634474363No Hit
AATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAA14860.1212392181946942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14840.12107604293467442No Hit
CTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGGATTCTC14780.1205865171546151No Hit
TGTCCATGGCATCCATAGCGCCTGTCTCTTATACACATCTCCGAGCCCAC14600.1191179398144371No Hit
GTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAAT14570.11887317692440742No Hit
GGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTG14370.11724142432420967No Hit
CTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAA14270.11642554802411079No Hit
ACAAAGAACCGCACTCCTCGCCACACGGGATTCTCACCCTCTATGACGTC13920.11356998097376467No Hit
CACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGG13920.11356998097376467No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC13890.11332521808373502No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTA13780.11242775415362624No Hit
CTCTATGACGTCCTGTTCCAAGGAACATAGACAAGGAACGGCCCCAAAGT13590.11087758918343837No Hit
CTTTACAAAGAACCGCACTCCTCGCCACACGGGATTCTCACCCTCTATGA13290.10842996028314171No Hit
ATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTA13290.10842996028314171No Hit
GTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTC13110.10696138294296373No Hit
GCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTC13000.10606391901285496No Hit
CCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCC12770.10418740352262752No Hit
GCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGT12710.10369787774256818No Hit
GGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTT12600.10280041381245941No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)