FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1451412
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC19638413.530548183424141No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC88790.6117491105213406No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG77050.530862360239546No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA55980.38569337996378694No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG35640.24555398467147851No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA29460.20297475837322554No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC24120.16618299972716224No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21960.15130094005010294No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG19710.13579879455316615No Hit
ACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCA18090.12463724979537168No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA17380.11974546166078275No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCAC16720.11519816564834796No Hit
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC16370.11278672079326889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16020.11037527593818984No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)