FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657702
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC29948518.06627487932089No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT273481.6497536951756104No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG251881.5194528328975896No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA110410.6660425094498287No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA95000.573082496130185No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA74140.4472456448746518No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCT68770.4148514027249771No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT55410.33425790642709No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG52990.31965938389408954No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC48140.29040201435481167No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA46050.2777941994399476No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA43160.2603604266629346No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG38860.23442090315388414No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT36210.2184349177355158No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT31280.18869495241002304No Hit
TGTCCATGGCATCCATAGCGCCTGTCTCTTATACACATCTCCGAGCCCAC30680.18507548401341134No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCT29450.17765557380035735No Hit
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC28590.17246766909854727No Hit
TGGTGTCTGTGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA27700.16709879097690658No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATG26560.16022180102334438No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCAC26270.1584723912983154No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA23750.14327062403254626No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC21940.13235189436943431No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT20420.12318257443135136No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20110.12131251575976865No Hit
ATGCACTGCAGGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGC18860.11377195660016094No Hit
AGGGCTTCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC18200.10979054136388809No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTC18060.10894599873801203No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)