FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192782
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC367273.0791041447640897No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT265802.228403849152653No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC142731.1966143016913402No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC91650.7683717561130199No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT77770.6520051442761544No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA66880.5607059798018413No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT58310.4888571423780708RNA PCR Primer, Index 27 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT54610.4578372242371196RNA PCR Primer, Index 27 (95% over 23bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG50970.4273203317957514No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA49630.41608609117173134No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA38460.3224394734326977No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA35130.29452154710584166No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA31960.26794502264454023No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT21820.18293367941501465No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC21040.17639434532043574No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT19830.16624999371217875No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT18270.15317132552302096No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC16870.14143405919941784No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC16870.14143405919941784No Hit
GTGTATAAGAGACAGCCAGGTCTGAAGGCAGTCTCTTATACACATCTCCG16500.13833206738532272No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA16110.13506240033803327No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG15640.13112203235796652No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC15450.12952911764262034No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA15390.12902609194303738No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA14570.12215140738206982No Hit
GCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTC14380.1205584926667237No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC13980.11720498800283706No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA13870.11628277422026825No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCC13390.11225856862360432No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC12650.10605458499541409No Hit
TCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC12410.10404248219708212No Hit
GTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCA12030.10085665276638983No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)