FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711870
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC88611.2447497436329666No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT63380.8903310997794541No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA25360.3562448199811763No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT24100.3385449590515122RNA PCR Primer, Index 1 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC23980.3368592580105918No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA23180.32562125107112255No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT20240.28432157556857296No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT14330.20130079930324357TruSeq Adapter, Index 1 (95% over 21bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA12560.1764367089496678No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT9140.12839422928343658No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG9030.12684900332925955No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC8960.125865677722056No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA8520.11968477390534787No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA8380.11771812269094076No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA7590.10662059083821483No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)