FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987775
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT256462.596340259674521No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC88230.8932196097289362No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC73940.7485510364202373No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT53450.5411151324947483TruSeq Adapter, Index 11 (95% over 21bp)
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA52200.5284604287413632No Hit
GTGTATAAGAGACAGCCAGGTCTGAAGGCAGTCTCTTATACACATCTCCG39910.40403938143808055No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA32870.33276808989901546No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT32320.32720002024752604RNA PCR Primer, Index 11 (95% over 23bp)
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT29300.29662625597934755No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA23030.23315026195236768No Hit
GTATAAGAGACAGCCAGGTCTGAAGGCAGTCTCTTATACACATCTCCGAG14420.1459846624990509No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT13730.1389992660271823No Hit
GTGTATAAGAGACAGCTGACACACATCTCCGAGCCCACGAGACCGTACTA12900.13059654273493457No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA10820.1095391156893017No Hit
GGACAAGGAGGAGTCCCTGTCTCTTATACCTGTCTCTTATACACATCTCC10320.10447723418794766No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)