FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004048636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543234
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC293105.395464937761628No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT148142.727001623609715No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG88411.627475452567402No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG54030.9945990125802141No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC51920.955757555675823No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA45410.835919695748057No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT31730.5840945154390189RNA PCR Primer, Index 17 (95% over 21bp)
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA24830.45707742887963565No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT20980.38620557623418267No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG20520.37773777046355717No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGT17020.3133088135131453No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA15400.28348741058181187No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA14670.27004937098929743No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT13550.24943210476516564RNA PCR Primer, Index 17 (95% over 23bp)
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC13530.2490639392968776No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG13200.24298920907012445No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC12280.22605359752887336No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC10970.20193875935600497No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCC10460.19255053991465923No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG9580.17635125930998427No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT8250.15186825566882778No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT8150.15002742832738747No Hit
GTGTATAAGAGACAGCCAGGTCTGAAGGCAGTCTCTTATACACATCTCCG8090.14892293192252326No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC7710.14192778802504996No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA7710.14192778802504996No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA7500.13806205060802526No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA7470.13750980240559316No Hit
TGGTGTCTGTGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA6780.12480809374965485No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA6550.12057419086434207No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG6400.11781294985218156No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG5680.10455899299381113No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)