FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537090
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC998456.495715930752266No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT456822.9719795197418497No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG163651.0646741570109752No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA117070.7616339967080652No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT91930.598078186703446No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC73060.47531374220117234No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT53020.34493751179176235No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT47440.3086351482346512No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA46660.3035606242965604No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC40250.26185844680532694No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA38100.24787097697597407No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC35030.22789817121964231No Hit
TGGTGTCTGTGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA34570.22490550325615288No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA33420.21742383334742924No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC32530.21163367141806919No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC31950.20786030746410425No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTA30690.1996629995641114No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA29960.19491376562205204No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA28860.1877573857093599No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT27350.17793362782920974RNA PCR Primer, Index 39 (96% over 25bp)
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC25270.16440156399430092No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC25030.1628401720133499No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA22900.14898281818240963No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT21740.1414360902744797RNA PCR Primer, Index 39 (95% over 23bp)
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC21060.13701214632845182No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA19880.1293353024221093No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG19700.12816425843639603No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTA16860.109687786661809No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT15920.10357233473641751No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)