FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135975
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC176481.553555315918044No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT168271.4812825986487377No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT84820.7466713616056692No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC67230.5918264046303836No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA58210.5124232487510729No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT47490.41805497480138204No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG40230.35414511763022954No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA39340.34631043816985413No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT32110.28266467131759065TruSeq Adapter, Index 8 (95% over 22bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA30830.27139681771165736No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA27920.24578005677941858No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC22710.19991637139901847No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC21920.19296199300160655No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC21670.19076124034419772No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT21500.18926472853715973No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT20270.17843702546270823No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT17270.15202799357380226No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA14740.12975637668082485No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA12290.10818900063821826No Hit
GTATAAGAGACAGTGTCCATGCATCCATAGCGTCTGTCTCTTATACACAT12180.10722066946895839No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)