FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419380
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC307512.1665093209711284No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG285852.013907480731023No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT219521.5465907649818935No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC85910.605264270315208No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA85840.6047710972396398No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG71260.5020501909284335No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA60510.42631289718045906No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA55450.3906635291465288No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT55050.387845397286139No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG48780.3436711803745297No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA47230.3327509194155195No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC42840.30182192224774196No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC42060.296326565119982No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT39290.27681100198678293TruSeq Adapter, Index 3 (95% over 21bp)
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC38860.273781510236864No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA33600.23672307627273878No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT30870.21748932632557877No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT25650.18071270554749255No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA23720.16711521932111204No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA22980.16190167537939099No Hit
GTATAAGAGACAGTGTCCATGCATCCATAGCGTCTGTCTCTTATACACAT18530.13054995843255504No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC17590.12392734856063917No Hit
TTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA17040.12005241725260324No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGTCGTATGCCGT16670.11744564528174274No Hit
GATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCG16550.1166002057236258No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA16270.11462751342135298No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG15570.10969578266567093No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC14850.10462314531696937No Hit
GGACTCCTCCTTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC14650.10321407938677452No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT14210.10011413434034579RNA PCR Primer, Index 3 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)