FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1789353
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC20572211.497004783293178No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC1441558.056263912151488No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG575943.2187053085668396No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA417552.3335250227316804No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT310351.734425795245544No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC200181.1187283895352118No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA171090.9561556607332371No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC124210.6941615209519866No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT123530.6903612646582312No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA113020.6316249504709245No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA109460.6117294910506759No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA103940.5808803517248973No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA95040.5311417031742758No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA91400.5107991547782914No Hit
TGGTGTCTGTGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA91190.5096255462169846No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA73870.41283078297015735No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC70790.39561785740432437No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC65100.3638186540051069No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC62500.3492882622936894No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC62470.34912060392778843No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCC60500.33811103790029134No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT57910.32363653231084083RNA PCR Primer, Index 27 (95% over 23bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC56490.31570070299152825No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT56040.31318582750301366No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT55390.3095532295751593No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC53810.3007232223043748No Hit
TTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA48260.26970642461269523No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCC44760.2501462819242486No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA44620.24936387621671074No Hit
GTATAAGAGACAGTGTCCATGCATCCATAGCGTCTGTCTCTTATACACAT43180.24131627465346414No Hit
TGTCCATGCATCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA41590.23243038126071267No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC39950.22326505725812623No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG31570.1764324870497884No Hit
TGGTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCG31350.1752029923665146No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGCATCCATAGCGTCTGTCTC31270.17475590339077868No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC31080.17369406707340584No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA29890.16704361855933403No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC29640.16564646551015927No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT29620.16553469326622527RNA PCR Primer, Index 27 (96% over 25bp)
TGGTGTCTGTGATGTTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA28630.16000196719149323No Hit
GATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCG28130.15720766109314374No Hit
GTGTATAAGAGACAGTGTCCATGCATCCATAGCGTCTGTCTCTTATACAC27890.15586639416593595No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG26270.1468128424072835No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT24650.1377592906486311No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC23810.1330648564034039No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT23480.13122061437849322No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACAC22870.12781156093850682No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG22760.12719681359686993No Hit
TGTCCATGGCATCCATAGCGCCTGTCTCTTATACACATCTCCGAGCCCAC22630.12647029401129906No Hit
CTCTAGAGACTGTCAGGGAATCAATGCACCGTTCCCTGACAGTCTCTAGA22190.12401130464475148No Hit
GTATCAACGCAGAGTACATGGGCACTCTAGAGACTGTCAGGGAACGGTGC21930.12255826547360973No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA21640.12093756793656703No Hit
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC20900.11680199491100973No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC20670.11551661410576897No Hit
GCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTC20370.11384003044675925No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAC20090.11227521903168353No Hit
TGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCG20000.11177224393398061No Hit
GGACTCCTCCTTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC19640.10976034354316895No Hit
TGTCCATGGCATCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG19410.10847496273792818No Hit
TGTCCATGGCATCTATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC18960.10596008724941361No Hit
GTATCAACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTC18750.10478647868810682No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCCTGTCTCTTAT17930.10020381668681361No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)