FastQCFastQC Report
Fri 9 Sep 2022
EGAF00004048666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2628873
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC795823.027228778263537No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG416741.585242040981059No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA162920.6197332469084661No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG158220.6018548632817181No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG101690.38681975127744855No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT89150.3391187021967208No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA80970.3080027068633593No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT57220.21765981087713254No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG50110.1906140007524137No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA48390.18407127312730587No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA46230.1758548244818217No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC41170.15660703274749294No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG34890.1327184690930296No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC32910.12518672450133575No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC31250.11887223156082473No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA30180.1148020463521821No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC30060.11434557698298851No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA27010.10274364718265204No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)