FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214921
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC291122.396205185357731No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT112410.9252453451705913No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG93250.7675396178023098No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT50520.41582950660989476RNA PCR Primer, Index 17 (95% over 23bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG39770.32734638713134434No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA35100.2889076738322903No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT34160.2811705452453287No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC33250.2736803462941212No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA30330.2496458617473893No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG28650.23581780214516007No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT25280.20807937306211677RNA PCR Primer, Index 17 (95% over 21bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG17270.14214916031577363No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA16920.1392683145653092No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA16320.1343297218502273No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT14250.11729157698319478No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG14080.11589230904725492No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA13740.11309377317537518No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA13560.11161219536085062No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG13470.11087140645358834TruSeq Adapter, Index 12 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGT12610.1037927568953043No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)