FastQCFastQC Report
Fri 9 Sep 2022
EGAF00004048686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2595820
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC613492.3633765053046822No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT532782.05245355995408No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC176520.6800163339522771No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT109950.4235655785069843No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT107270.41324128791672765RNA PCR Primer, Index 27 (95% over 23bp)
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA92640.35688144786618486No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT89390.34436131935188113RNA PCR Primer, Index 27 (96% over 25bp)
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA67380.25957115670578085No Hit
GTGTATAAGAGACAGCCAGGTCTGAAGGCAGTCTCTTATACACATCTCCG66000.2542549175212457No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA61920.23853734080175049No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT58860.2267491582621291No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG48630.18733964604633604No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA48460.18668474701635707No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC48030.1850282377052338No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA39700.15293818523626446No Hit
GTGTATAAGAGACAGCTGACACACATCTCCGAGCCCACGAGACGGACTCC39160.15085791772927246No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA37550.14465563868064812No Hit
GGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATA31290.12053994498848147No Hit
GGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGAGCCATTC30100.11595565177862871No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT29750.11460733024631908No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG27930.10759605827830897No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG27810.10713377661008854No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC27660.10655592452481297No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26490.1020486782596636No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG26470.1019716313149602No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)