FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004048689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2378150
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC802173.373084120009251No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT273871.15160944431596No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC168450.7083236969913589No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA152720.6421798456783635No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT141470.5948741668944347No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA119340.5018186405399155No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA110600.46506738431133443No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA105880.4452200239682106No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC105210.4424027079873011No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG98990.41624792380632003No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT63750.26806551310892923TruSeq Adapter, Index 10 (95% over 21bp)
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT54800.23043121754304816No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA54520.2292538317599815No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA39030.16411916826104325No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT37630.1582322393457099No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC37500.15768559594642895No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT33890.1425057292433194No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA31440.13220360364148603No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT30780.1294283371528289RNA PCR Primer, Index 10 (95% over 23bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT28960.12177532956289554No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC28960.12177532956289554No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA27580.11597249963206695No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCCTGTCTCTTAT25490.1071841557513193No Hit
GCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTC23950.10070853394445262No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)