FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004048694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41876
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT28256.746107555640463No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC13443.209475594612666No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC10792.5766548858534724No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA6291.502053682300124No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT6271.4972776769509981No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT4971.1868373292578087TruSeq Adapter, Index 1 (95% over 22bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA4160.9934091126182062No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA3910.9337090457541313No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCT3090.7378928264399657RNA PCR Primer, Index 1 (95% over 24bp)
TGTCCATGGCATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAG2860.6829687649250167No Hit
GTGTATAAGAGACAGCCAGGTCTGAAGGCAGTCTCTTATACACATCTCCG2470.58983666061706No Hit
GGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAT2020.4823765402617251No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG2010.47998853758716203No Hit
TGTCCATGGCATCCATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1860.44416849746871717No Hit
GTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCA1830.43700448944502823No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA1630.3892444359537683No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT1600.3820804279300793No Hit
CATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT1520.3629764065335753No Hit
TCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA1410.33670837711338236No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA1390.3319323717642564No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG1380.32954436908969337No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT1320.31521635304231543No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA1220.29133632629668543No Hit
GCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTC1210.2889483236221224No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGT1080.25790428885280353No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCCTGTCTCTTAT1030.24596427547998856No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG1010.24118827013086255No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT1000.23880026745629954No Hit
ACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGC1000.23880026745629954No Hit
CCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG970.23163625943261054No Hit
GCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC900.2149202407106696No Hit
GTATAAGAGACAGCCAGGTCTGAAGGCAGTCTCTTATACACATCTCCGAG890.21253223803610657No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATGTCGTATGCCGT860.2053682300124176No Hit
ATGCCCTGCAGTGCATCTGTCTCTTATACCTGTCTCTTATACACATCTCC810.19342821663960263No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATGTCGTATGCC800.19104021396503965No Hit
TGTCCATGGCATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACG790.18865221129047666No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG730.17432419524309867No Hit
TGTCCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG690.16477218454484668No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGAGCTCGTATGCCGT680.1623841818702837No Hit
GACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAG660.15760817652115772No Hit
GGACAAGGAGGAGTCCCTGTCTCTTATACCTGTCTCTTATACACATCTCC660.15760817652115772No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACCTGTCTCTTATACACA650.1552201738465947No Hit
GTATCAACGCAGAGTACATGGGGCAGGCCTCTGCAGTCGACGGGCCCGGT650.1552201738465947No Hit
GGACAAGGAGGAGTCCCTGTCTCTTATACACCTGTCTCTTATACACATCT630.15044416849746872No Hit
TGTCCATGGCATCCATAGCGTCTGTCCTGTCTCTTATACACATCTCCGAG630.15044416849746872No Hit
TGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATC620.14805616582290573No Hit
TGTCCATGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA620.14805616582290573No Hit
GAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCG610.14566816314834272No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCTGTCTCTTATACACATCT580.13850415512465375No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGGCGT570.13611615245009073No Hit
TGTCCATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG550.13134014710096475No Hit
GTGTATAAGAGACAGACGCTATGGATGCCATGGACACTGTCTCTTATACA520.12417613907727577No Hit
ATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC520.12417613907727577No Hit
ATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATC510.12178813640271277No Hit
GTATAAGAGACAGTGTCCATGCATCCATAGCGTCTGTCTCTTATACACAT510.12178813640271277No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTG510.12178813640271277RNA PCR Primer, Index 40 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCT500.11940013372814977RNA PCR Primer, Index 1 (95% over 21bp)
GTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCG480.11462412837902379No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACC470.11223612570446079No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGCATCCATAGCGTCTGTCTC470.11223612570446079No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC450.1074601203553348No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC450.1074601203553348No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.1050721176807718No Hit
GTGTATAAGAGACAGACGCTATGGATGCCATGGACACTGTCTCTTATACC440.1050721176807718No Hit
GCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCT430.1026841150062088No Hit
CGTCAGATGTGTATAAGAGACAGATGCACTGCAGGTACTTGACTGTCTCT430.1026841150062088No Hit
TGTCCATGGCATCCATAGCGTCCTGTCTCTTATACACATCTCCGAGCCCA420.10029611233164581No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)