FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1995859
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC1325926.6433550666655306No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT463722.3234106216922137No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG196850.9862921178299668No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA161640.8098768500179623No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT113350.5679258905563971No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA83700.41936830206943476No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC69680.3491228588793096No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT67090.33614599027285996No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA49760.24931620921117173No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA48860.24480687262977996No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC46420.23258156012022896No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC41330.2070787565654688No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT40310.20196817510655815No Hit
TGGTGTCTGTGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA38580.19330022812232728No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC36540.18307906520450593No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC36220.1814757455311222No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTA35170.17621485285283178No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA33380.1672462834298415No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC33220.1664446235931496No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA33140.16604379367480368No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA31950.1600814486394079No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC26840.13447843760506129No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT22250.11148082103996325RNA PCR Primer, Index 39 (95% over 23bp)
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT21640.10842449291257548No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)